![]() Infected muskmelon plants were collected and a fungus was isolated during field survey of muskmelon conducted in 4th, 5th, and 6th agroclimatic zones of Karnataka state. This study identified the Jeonbuk isolate to be D. Phylogenetic analysis, based on rDNA ITS sequence, clearly distinguished D. bryoniae is a monophyletic group distinguishable from other Didymella spp., including Ascochyta pinodes, Mycosphaerella pinodes, M. Phylogenetic analysis with related species of D. Nucleotide sequences of the rDNA ITS region from pure culture ranged from 98.2% to 99.8%. ![]() The similarity in sequence identity between the rDNA ITS region of the Jeonbuk isolate and other Didymella bryoniae from BLAST searches of GenBank was 100% and was 95.0% within the group. Morphological examination of the Jeonbuk isolate revealed that the percentage of monoseptal conidia ranged from 0% to 10%, and the average length × width of the conidia was 70 (± 0.96) × 32.0 (± 0.15) µm on potato dextrose agar. In this study, morphological characteristics and rDNA internal transcribed spacer (ITS) sequences were analyzed to identify the causal organism of this disease. Gummy stem blight is a major foliar disease of muskmelon (Cucumis melo L.). All the cucurbitaceous hosts screened, developed characteristic symptoms of the disease and were found to be susceptible. Pathogenicity tests were carried out on crops belonging to the cucurbit family utilizing representative cultured isolates. Of the isolations done, a total of 12 pathogen isolates were obtained which were all identified as Didymella bryoniae according to spore morphology and cultural characteristics. It was observed that gummy stem blight had a greater incidence during the wet as opposed to the dry season and also in comparison to all other pumpkin foliar diseases observed. Field data on disease incidences were collected in Trinidad, along with sample isolations and confirmation of the pathogens through morphological characterization. Symptoms can occur on either the leaf, stem and or fruit of the plants, as drying of plants leading to premature death and significantly decreased yields of the crop. This disease is caused by Didymella bryoniae which is known to target most cucurbit crops. Pumpkin fields in Trinidad have been noted to become increasingly afflicted by gummy stem blight.
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